Olteo.com – extravirgin olive oil -Olives – Extra Virgin Olive Oil – Extravirgin Olive Oil – Italian Olive Oil – Olive Oil

Anti-Atherogenicity of Extra Virgin Olive Oil and its Enrichment with Green Tea Polyphenols in the Atherosclerotic Apolipoprotein E-Deficient Mice: Enhanced Macrophage Cholesterol Efflux

Mira Rosenblat 1, Nina Volkova 1, Raymond Coleman 2, Yaron Almagor 3, Michael Aviram 1

1 The Lipid Research Laboratory, Technion Faculty of Medicine, The Rappaport Family Institute for Research in the Medical Sciences, Rambam Medical Center,.
2 Department of Anatomy and Cell Biology, Technion Faculty of Medicine, Haifa, Israel
3 Department of Cardiology, Shaarei Zedek Medical Center, Jerusalem, Israel

Running Head: Olive oil anti-atherogenicity
Key words: olive oil, green tea polyphenols, macrophages, oxidative stress, atherosclerosis
• Address correspondence to: Prof. Michael Aviram, D.Sc. The Lipid Research Laboratory, Rambam Medical Center, Haifa, Israel, 31096.
Fax: 972-4-8542130; Tel: 972-4-8542970; E mail: aviram@tx.technion.ac.il

Abstract
The anti-atherogenic properties of extra virgin oil (EVOO) enriched with green tea polyphenols (EVOO-GTPP), in comparison to EVOO, were studied in the atherosclerotic apolipoprotein E-deficient (E0) mice.
E0 mice (8 mice in each group) consumed EVOO or EVOO-GTPP (7µl/mouse/day, for 2 months) by gavages feeding. The placebo group received only water. At the end of the study blood samples, peritoneal macrophages and aortas were collected.
Consumption of EVOO or EVOO-GTPP resulted in a minimal increase in serum total and HDL- cholesterol levels (by 12%), and in serum paraoxonase 1 activity (by 6% and 10%).
EVOO-GTPP (but not EVOO) decreased the susceptibility of the mice serum to AAPH-induced lipid peroxidation (by 18%), compared to the placebo-treated mice.  The major effect of both EVOO and EVOO-GTPP consumption was on high density lipoprotein (HDL)-mediated macrophage cholesterol efflux.
E’ stato altresì osservato che l’assunzione di EVOO-GTPP (ma non di EVOO) diminuiva (del 18%) la risposta del siero degli animali quando sottoposti a perossidazione lipidica indotta da AAPH se comparata al gruppo di controllo (placebo).
Consumption of EVOO stimulated cholesterol efflux rate from MPM by 42%, while EVOO-GTPP, increased it by as much as 139%, compared to the placebo-treated mice MPM.
Finally, the mice atherosclerotic lesion size was significantly reduced by 11% or 20%, after consumption of EVOO or EVOO-GTPP, respectively.
We thus conclude that EVOO possesses beneficial anti-atherogenic effects and its enrichment with green tea polyphenols further improved these effects, leading to the attenuation of atherosclerosis development.
ScienceDirect

1.Antiatherogenicity of extra virgin olive oil and its enrichment with green tea polyphenols in the atherosclerotic apolipoprotein-E-deficient mice: enhanced macrophage cholesterol efflux
The Journal of Nutritional Biochemistry, In Press, Corrected Proof, Available online 27 September 2007
Mira Rosenblat, Nina Volkova, Raymond Coleman, Yaron Almagor and Michael Aviram